This section will let you about the major difference existing between the 16s rRNA and 16s DNA sequencing.
Differences That One Should Know About DNA Sequencing
- 16s rRNA is considered to be a component of the 30S subunit or small subunit in the prokaryotic ribosome. On the other hand, the 16S rDNA includes the gene that codes 16S rRNA.
- Again, the 16S rDNA takes part in the process of transcription for the production of its gene product which is known to be the 16S rRNA.
Whereas, the 16S rRNA is known to participate in the binding to the Shine-Dalgarno sequence which is present on the mRNA to be translated.
- The prokaryotes usually possess multiple 16S rDNA sequences per genome. On the other hand, the 16S rDNA is usually conserved among the different species of archaea as well as bacteria.
So, both the 16S rDNA and also the 16S rRNA is known to be the two different types of nucleotide sequences that occur in prokaryotes. As a result, they are considered to be responsible for the facilitation of the prokaryotic mRNA translation.
More About 16S rDNA
If you want to get a brief idea about the 16s DNA sequencing, then you should first know about the 16S rDNA well.
The 16S rDNA is the particular gene that encodes 16S rRNA in the prokaryotes. In this case, a majority of the functionality-related prokaryotic genes are organized into operons. Due to this particular reason, 16S rDNA mainly occurs in an operon along with the other two genes which are considered to be responsible for the synthesis of ribosomes.
So, the other genes involve 5S rDNA and 23S rDNA genes. Also, there can be the presence of more than one copy of the 16S rDNA in a bacterium genome.
This 16S rDNA is generally found in different prokaryotes in the form of a conserved DNA sequence. Because of this particular reason, the prokaryotes which contain similar 16S rDNA sequences are considered to be phylogenetically similar.
More About 16S rRNA
16S rRNA is considered to be a type of rRNA that is responsible for constituting the small subunit of the prokaryotic ribosome. The structural role of this is identical to the rRNA specifically in the large subunit.
However, it also interacts with the 23S rRNA in the large subunit by facilitating the binding of the small subunit to the large subunit.
So, this 16S rRNA is known to be highly specific as well as conserved. Also, the gene sequence, in this case, is considered to be large enough.
Also, this 16S rRNA gene sequencing is mainly used for the classification, identification as well as quantitation of microbes within a complex biological mixture.
So, the above-discussed ones are considered to be some of the major differences existing between the 16s rDNA and 16s rRNA sequencing that you should know about.
Also, you will find that the major difference existing between the 16S rDNA as well as 16S r RNA lies in their function.